Introduction Pythiosis, caused by Pythium insidiosum ( P. insidiosum ), is an emerging disease with high mortality and morbidity. Despite its clinical severity and geographical strain variations, diagnostic and treatment challenges persist. Materials and methods We queried PubMed and Google Scholar for “ P. insidiosum ” and “pythiosis,” 1084 human pythiosis cases to generate a corresponding distribution map. Molecular biology, morphology, modified sporulation technique, and microscopic observation were employed to understand the biological properties of P. insidiosum with a strain of P. insidiosum isolated from a patient in Guangzhou. Drug susceptibility studies on the isolate were conducted both in vitro and in vivo . Results Molecular biology, morphology, and biological processes confirmed that this strain was P. insidiosum . Epidemiological investigations have revealed that India and Thailand are hotspots for human pythiosis, and sporadic cases are increasing in China, the Americas, and Europe. The modified method of zoospore induction achieved 250-fold greater than the traditional method within 24 h. In vitro drug testing demonstrated superior antibiotic sensitivity (doxycycline (DOX) MIC 4 μg/mL; azithromycin (AZM) MIC 8 μg/mL) versus antifungals ( 128 μg/mL). Subcutaneous infection models in immunocompromised mice showed 80 and 90% survival with oral AZM/DOX monotherapy versus 20% in controls ( P 0.05), correlating with reduced hepatic fungal burdens and attenuated neutrophilic periarteritis. Conclusion The distribution map underscores pythiosis as a growing climate-sensitive disease that requires enhanced surveillance in non-endemic regions. The modified method of zoospore induction and microstructure observation with Transmission Electron Microscopy (TEM) may be helpful for the rapid and sensitive detection of P. insidiosum . We also provided theoretical and technical support for the effective treatment of pythiosis in humans and animals with DOX and AZM in China, which offers novel insights into the potential of certain antibiotics as effective treatments.
Liu et al. (2025) studied this question.