This study systematically compares three unsupervised segmentation algorithms (Simple Linear Iterative Clustering (SLIC), Felzenszwalb’s graph-based method, and the Watershed algorithm) in combination with two classification approaches: Random Forest using histogram-based features and Convolutional Neural Networks (CNNs). The study employs Bayesian optimization to jointly tune segmentation parameters and model hyperparameters, investigating how segmentation quality impacts downstream classification performance. The methodology is validated using light optical microscopy images of a high-strength steel sample, with performance evaluated through stratified cross-validation and independent test sets. The findings demonstrate the critical importance of segmentation algorithm selection and provide insights into the trade-offs between feature-engineered and end-to-end learning approaches for microstructure analysis.
Hallo et al. (2026) studied this question.