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January 26, 2026JCO oncology advances.0 citationsOpen Access

Chemoradiation and Image-Guided Brachytherapy Alone or in Combination With Protease Inhibitor (Nelfinavir Mesylate) in Stage III Cervical Cancer: Results From a Phase III Trial

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SCS. ChopraPMPrachi MittalAGAnkita Gupta

Key Points

  • This trial aimed to evaluate the effectiveness of nelfinavir, an AKT pathway inhibitor, alongside chemoradiation and brachytherapy in improving disease-free survival for patients with stage III cervical cancer.
  • Conducted a phase III adaptive trial (NELCER) with random assignment of patients to two treatment arms.
  • Patients received either standard treatment (chemoradiation and brachytherapy) or standard treatment plus nelfinavir.
  • Nelfinavir was administered at 1,250 mg twice daily, starting 7-10 days before treatment and during chemoradiation.
  • The primary endpoint was disease-free survival (DFS) at three years, with secondary endpoints including overall survival and adverse events.
  • At 34.9 months follow-up, the 3-year DFS was 71% in the standard arm versus 56% in the nelfinavir arm (P = .40).
  • The 3-year disease-specific survival (DSS) was 82% in the standard arm and 57% in the nelfinavir arm (P = .09).
  • Nelfinavir was associated with a higher incidence of acute grade ≥ 3 gastrointestinal effects (8.7% vs. 0%, P = .04).
  • Due to lack of clinical efficacy and higher adverse events, the trial was halted after interim analysis.

Abstract

PURPOSE The AKT pathway is upregulated in cervical cancer. This trial aimed to investigate whether the protease AKT pathway inhibitor (nelfinavir mesylate), in addition to chemoradiation (CRT) and image-guided brachytherapy (IGBT), could improve disease-free survival (DFS). METHODS NELCER (ClinicalTrials.gov identifier: NCT03256916 ), a phase III adaptive trial, randomly assigned patients with stage III cervical cancer (International Federation of Gynecology and Obstetrics 2018) to CRT and IGBT (standard arm) or in combination with nelfinavir (test arm). Patients in the test arm received nelfinavir 1,250 mg twice a day starting 7-10 days before and during CRT. The primary end point was 3-year DFS. Secondary end points included locoregional control, overall survival, adverse events, and quality of life. Disease-specific survival (DSS) was also estimated. The study planned to randomly assign 348 patients to detect a hazard ratio (HR) of 0.66. Futility analysis was scheduled at 32 events. RESULTS Ninety-two patients were recruited at the time of futility analysis. The stage was IIIA-B (43.5%), IIIC1 (48.9%), and IIIC2 (7.6%) patients. The median prescribed dose to high-risk clinical target volume was 88 Gy 10 (IQR, 83.6-92 Gy 10 ). At a median follow-up of 34.9 months (29-40.8), the 3-year DFS was 71% (95% CI, 0.58 to 0.86) in the standard arm and 56% in the nelfinavir arm (95% CI, 0.43 to 0.70; HR, 1.34 0.66 to 2.7; P = .40). The nelfinavir arm had a higher incidence of acute grade ≥ 3 (8.7% v 0%, P = .04) GI effects. The 3-year DSS was 82% (95% CI, 0.70 to 0.95) in the standard arm and 57% (95% CI, 0.43 to 0.75) in the nelfinavir arm (HR of 1.97 0.87 to 4.4, P = .09). The z-value was 0.74. CONCLUSION The 3-year DFS did not cross prespecified statistical boundary; however, on the basis of DSS and acute adverse events, nelfinavir was not considered clinically efficacious, and the trial was stopped at the first interim analysis.

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Cite This Study

Chopra et al. (2026) studied this question.

synapsesocial.com/papers/6977032e722626c4468e83fdhttps://doi.org/10.1200/oa-25-00088
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