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February 8, 2026New Zealand Journal of Crop and Horticultural Science0 citations

Molecular and Functional Characterization of Halophilic Plant Growth Promoting Rhizobacteria From Suaeda fruticosa L. Rhizosphere Using 16s rDNA Sequencing

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SNSomu NagajyothiMKMonika KayasthHMHemanthkumar Manne

Key Points

  • The aim is to isolate and characterize plant growth-promoting salt-tolerant bacteria from the rhizosphere of Suaeda fruticosa.
  • Collected rhizospheric soil samples from Suaeda fruticosa.
  • Isolated and screened bacteria for salt tolerance at various NaCl concentrations.
  • Assessed plant growth-promoting traits of salt-tolerant isolates.
  • Characterized isolates biochemically and using 16S rDNA sequencing.
  • Eighteen bacteria were isolated, twelve were confirmed as salt-tolerant.
  • Isolates SA3, SR1, SR2, and SS1 produced the highest indole acetic acid.
  • Isolate SR1 showed the highest ammonia production.
  • Ten isolates demonstrated phosphate solubilization, and eight showed zinc solubilization.
  • SA3 and SR1 were identified as Bacillus species with high similarity to known strains.

Abstract

The present investigation was undertaken to isolate and characterize plant growth‐promoting (PGP) salt‐tolerant bacteria. Rhizospheric soil samples were collected from Suaeda fruticosa halophyte in Hisar and were screened for PGP salt‐tolerant bacteria. Eighteen isolates were retrieved and analyzed for salt tolerance at various NaCl concentrations (2, 4, 6, 8, 10% NaCl). Twelve isolates were subsequently assessed for their PGP traits after demonstrating salt tolerance across varying NaCl concentrations. Highest amount of Indole acetic acid was produced by isolates SA3, SR1, SR2, and SS1. Among the strains that were tested, isolated SR1 demonstrated the highest level of ammonia production. Ten isolates showed phosphate solubilization, and eight isolates showed Zn solubilization. All twelve salt‐tolerant bacterial isolates were found to be negative for siderophore production. Four isolates, SA3, SR1, SS1, and SR2, with significant salt tolerance and plant‐growth‐promoting traits, were characterized biochemically. Based on biochemical results, the tentative genus was identified as Bacillus (SA3, SR1, and SR2) and Serratia (SS1). The tentative taxonomic classification of isolate SA3 and SR1 is supported by their 99.64% similarity to Bacillus safensis and 99.78% similarity to Bacillus cereus as determined by 16S rDNA sequencing.

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Cite This Study

Nagajyothi et al. (2026) studied this question.

synapsesocial.com/papers/698828b90fc35cd7a88486a0https://doi.org/10.1002/nzc2.70105
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