The study aims to explore how G4-ligands can be used to precisely target G-quadruplex DNA structures in gene-regulatory regions.
Analyzed the binding affinity of G4-ligands to G-quadruplex DNA
Developed oligonucleotides conjugated with G4-ligands
Utilized biochemical assays to measure stabilization effects
G4-ligands increased binding to G-quadruplex structures significantly
Conjugated oligonucleotides showed enhanced specificity for target regions
Stabilization of G4 DNA resulted in altered gene expression profiles
Abstract
The non-B DNA secondary structure known as G-quadruplex (G4) DNA can form in gene-regulatory regions of the human genome. Organic small molecules, so called G4-ligands, which bind and stabilize G4...