Protocol for single-use Ambu aScope-based minimally invasive bronchoalveolar lavage collection in pigs

Here, we present a protocol for collecting bronchoalveolar lavage (BAL) samples from live pigs using a single-use Ambu aScope. We describe the steps for animal preparation, BAL collection, and sample handling, including downstream analysis, to ensure consistent, high-quality results. This approach applies to a wide range of respiratory research, including the evaluation of inflammatory responses and the testing of new therapies in clinically relevant pig models.