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February 14, 2026PeerJOpen Access

Gene mapping and development of molecular markers for thousand-grain weight in rye based on bulked segregant analysis

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Authors

LWLai WeiZZZhenbo ZhaiYYYunjie Yang

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Overview

Bulk segregant analysis identifies genomic regions for thousand-grain weight in rye, suggesting future breeding strategies.

Key Points

  • This research aims to map genes controlling thousand-grain weight in rye and develop molecular markers for these genes.
  • Performed bulk segregant analysis sequencing on a hybrid population of cultivated and wild rye.
  • Identified candidate regions containing single nucleotide polymorphisms across rye chromosomes.
  • Developed molecular markers based on the mapped genes with PCR validation.
  • Identified 10 candidate regions with 68 SNP loci across rye chromosomes.
  • Successfully designed six high-quality KASP markers and one SSR marker for TGW.
  • Single KASP marker TGW-16 was highly effective for selecting germplasm with superior TGW.

Cite This Study

Wei et al. (2026) studied this question.

synapsesocial.com/papers/699011032ccff479cfe57629https://doi.org/10.7717/peerj.20811
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