Introduction Arboviral diseases transmitted by the Aedes aegypti mosquito cause high morbidity and mortality worldwide. Objective To evaluate the repellent and insecticidal efficacy of water‐soluble formulations of essential oils impregnated into mosquito nets against the adult stage of A. aegypti . Methodology A randomized laboratory bioassay was conducted using a controlled factorial design to evaluate four essential oil formulations ( Eucalyptus globulus, Cymbopogon citratus, Origanum vulgare, and Mentha piperita ) at concentrations of 10, 100, and 250 mg/mL. Each experimental group consisted of 60 laboratory‐reared adult female A. aegypti specimens. The repellent effect, protection time, and insecticidal effect were evaluated by exposing the insects to a sedated animal bait ( Rattus norvegicus albinus ) covered with a mosquito net impregnated with the formulations of essential oils and DEET, as a positive control. Results The highest efficacy was seen in C. citratus, E. globulus , and O. vulgare at 250 mg/mL, with 93.3%–100.0% repellent effect and 180 min of protection time, similar to that obtained by 100 mg/mL DEET. The highest insecticidal effect was observed in C. citratus (26.7%) at a concentration of 250 mg/mL, similar to that observed in 100 mg/mL DEET. The repellent effect and protection time varied for each concentration tested, except for the insecticide, in which concentrations of 10 and 100 mg/mL had a similar effect (0%–6%). Conclusions The repellency of water‐soluble formulations of essential oils of C. citratus , E. globulus , and O. vulgare at 250 mg/mL, compared to 100 mg/mL DEET, represents a possible and complementary alternative for mosquito control.
Silva-Díaz et al. (2026) studied this question.