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February 21, 2026Journal of Molecular BiologyOpen Access

Importance of riboswitch-regulated genes for Pseudomonas aeruginosa

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Authors

SJShirin JahangirnejadJCJean-Philippe CôtéDLDaniel A. Lafontaine

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Overview

Analysis reports the essentiality of riboswitch-controlled genes in Pseudomonas aeruginosa, suggesting targets for new antibiotics.

Key Points

  • This research aims to evaluate the role of riboswitch-regulated genes in the survival of Pseudomonas aeruginosa under nutrient-limited conditions.
  • Assessed essentiality of riboswitch-controlled genes using a transposon library in Pseudomonas aeruginosa.
  • Conducted RT-qPCR assays to measure mRNA levels influenced by SAH.
  • Performed structural analyses to observe the riboswitch's conformational changes upon SAH binding.
  • Majority of riboswitch-controlled genes were found not essential for Pseudomonas aeruginosa growth.
  • Inactivation of the sahH gene resulted in complete growth failure under nutrient-limited conditions.
  • Supplementing S-adenosyl-methionine rescued growth despite sahH inactivation.
  • mRNA levels increased with SAH presence, indicating additional regulatory mechanisms.
  • Riboswitch showed significant rearrangement and affinity for SAH in structural analyses.

Cite This Study

Jahangirnejad et al. (2026) studied this question.

synapsesocial.com/papers/69994ba9873532290d01fc3chttps://doi.org/10.1016/j.jmb.2026.169724
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