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February 26, 2026Foods0 citationsOpen Access

Neutral Electrolyzed Water as Sanitizer Solution in Fresh Foods: The Strawberry as a Study Model

JRJuan C. Ramírez-OrejelPVPatricia Ventura-TorresKAKaren Arisbeth Alvarez-Cruz

Key Points

  • The study aims to evaluate the effectiveness of neutral electrolyzed water as a sanitizer for strawberries contaminated with harmful bacteria.
  • Strawberries contaminated with Salmonella Typhimurium and Escherichia coli were treated with neutral electrolyzed water.
  • Physicochemical characteristics of treated strawberries were analyzed.
  • Sensory analysis was conducted to assess the impact of treatments on fruit quality.
  • Comparisons were made with NaClO and saline solutions.
  • NEW reduced Salmonella Typhimurium counts to 1.9 log CFU/mL compared to 1.29 log CFU/mL for NaClO.
  • E. coli counts were reduced to 2.12 log CFU/mL with NEW, while NaClO achieved 1.31 log CFU/mL.
  • Physicochemical properties did not show statistically significant changes after treatment.
  • Sensory analysis indicated that NEW-treated strawberries maintained desirable attributes without off-odors.

Abstract

Strawberries are a very fresh product, but they pose food safety concerns. In the present work, the conservative effect of Neutral Electrolyzed Water (NEW) on strawberries was evaluated on contaminated berries with Salmonella Typhimurium ATCC 13311 and Escherichia coli O157:H7. In addition, the treated fruit underwent physicochemical analysis, evaluating color, pH, titrable acidity, reducing sugar, soluble solids, total phenolic compounds, and vitamin C content. The effects of NEW on strawberries were compared with those caused after treatment with NaClO and saline solutions; all evaluated solutions were applied by spraying. Subsequently, sensory analysis was performed to identify differences between treatments. NEW and NaClO showed similar in vitro bactericidal effects against E. coli, and for Salmonella Typhimurium, NEW and NaClO decreased their counts to 9.3 log CFU/mL and 5.16 log CFU/mL, respectively. When both solutions were sprayed on contaminated strawberries, S. Typhimurium counts were decreased to 1.9 log CFU/mL and 1.29 log CFU/mL with NEW and NaClO, respectively, and the E. coli counts were 2.12 log CFU/mL and 1.31 log CFU/mL, respectively. This work includes physicochemical properties of treated strawberries and a sensory study to identify how treatments affect fruit characteristics. The physicochemical properties of the treated strawberries were not affected statistically significantly. Sensory analysis revealed that although oxidizing sanitizers (NEW or NaClO) modified the overall sensory profile of strawberries, NEW-treated samples preserved key desirable attributes such as uniform appearance, fresh odor intensity, and characteristic strawberry flavor, without generating pronounced off-odors or unacceptable taste alterations. These results indicate that NEW is a promising postharvest sanitizing alternative capable of ensuring microbial safety while maintaining sensory quality relevant to consumer acceptance.

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Cite This Study

Ramírez-Orejel et al. (2026) studied this question.

synapsesocial.com/papers/699fe39d95ddcd3a253e7938https://doi.org/10.3390/foods15050800
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