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February 27, 2026Discover Food0 citationsOpen Access

Influence of bitter gourd (Momordica charantia L.) seed powder on physicochemical, phytochemical, and sensory properties of biscuits

AIAdeeba IlyasTKTusneem KausarMBMuhammad Bilal

Key Points

  • To examine how incorporating bitter gourd seed powder affects the physicochemical, phytochemical, and sensory properties of wheat-based biscuits.
  • Incorporation of bitter gourd seed powder at 0%, 5%, 10%, 15%, and 20% by weight in biscuit formulation.
  • Proximate analysis for protein, fiber, ash, fat, and carbohydrate content.
  • Physical tests measuring spread ratio, thickness, and diameter of biscuits.
  • Functional analysis measuring total phenolic and flavonoid contents, and antioxidant activity.
  • Sensory analysis using a 9-point hedonic scale with 30 panelists.
  • Protein, crude fiber, ash, and fat content in biscuits significantly increased with higher BGSP levels.
  • Physical characteristics (spread ratio, thickness, diameter) decreased as BGSP content increased.
  • Total phenolic content ranged from 0.218 to 1.244 mg/g GAE; total flavonoid content ranged from 0.109 to 0.699 mg/g CE; antioxidant activity increased from 0.099 to 0.265 µmol/g TE.
  • Biscuits with up to 5% BGSP received similar sensory scores to the control, while 10% and 15% had lower scores due to increased bitterness.

Abstract

Bitter gourd (Momordica charantia L.) seeds are usually thrown away as agro waste even though these are good source of protein, fiber, minerals as well as bioactive compounds with antioxidant activity. Utilization of these valuable waste materials in traditional food systems could create sustainable innovation, meeting UN Sustainable Development Goals (SDG 2, SDG 3 and SDG 12). The present study investigated the effect of incorporation of bitter gourd seed powder (BGSP) on the physicochemical, functional, antioxidant and sensory values, and storage stability of wheat-based traditional biscuits. BGSP was added at the 0% (control), 5, 10, 15 and 20% (w/w) by substituting refined wheat flour. Proximate analysis revealed that protein, crude fiber, ash, and fat content significantly (p < 0.05) rose with an increase in BGSP, however carbohydrate content decreased. Physical tests revealed that the spread ratio, thickness, and diameter decreased as the level of BGSP increased. Functional analysis showed that the total phenolic (TP) content (0.218–1.244 mg/g GAE), total flavonoid (TF) content (0.109–0.699 mg/g CE) and antioxidant activity (DPPH activity 0.099–0.265 µmol/g TE) significantly increased with the addition of BGSP. Sensory analysis (n = 30 panelists, 9-point hedonic scale) indicated that the incorporation of biscuits with up to 5% BGSP was well accepted with similar score of appearance, color, texture, and taste, to control, whereas 10% and 15% incorporation levels scored significantly less, based on increased bitterness and dark color. Comprehensively, the findings indicated that the incorporation of the BGSP up to 20% enhances the nutritional value and functionality of biscuits. The findings highlighted the potential to raise bitter gourd seeds as a cheap and viable food ingredient in the bakery sector.

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Cite This Study

Ilyas et al. (2026) studied this question.

synapsesocial.com/papers/69a134dded1d949a99abe48fhttps://doi.org/10.1007/s44187-026-00899-9
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