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Synapse
February 28, 20260 citations

Establishing Bacterial–Fungal Cross-Kingdom Biofilms for Studying Oral Pathogenesis

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Key Points

  • To model bacterial-fungal interactions in oral biofilms to understand their role in oral diseases.
  • Cultivated Streptococcus mutans and Candida albicans together under oral-like conditions.
  • Used confocal microscopy to monitor biofilm formation and behavior.
  • Assessed microbial interactions and their impacts on virulence.
  • Successful formation of cross-kingdom biofilms was achieved.
  • Observations showed enhanced microbial synergy in the biofilm.
  • Acidogenic virulence indicators were observed in biofilm environments.

Abstract

Source: Schlafer, S, and Frost Kristensen, M. Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy. J. Vis. Exp. (2020)This video demonstrates the cultivation of Streptococcus mutans and Candida albicans cross-kingdom biofilms under oral-like conditions to model bacterial–fungal interactions, enabling the study of microbial synergy, glucan-mediated adhesion, and acidogenic virulence in oral pathogenesis.

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Cite This Study

TODO TODO (2026) studied this question.

synapsesocial.com/papers/69a286370a974eb0d3c00fddhttps://doi.org/10.3791/24451
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