Comparative evaluation of antinuclear antibody detection by indirect immunofluorescence and line immunoassay with clinical correlation in suspected autoimmune disease patients: a retrospective cross-sectional study

Key Points

• Indirect immunofluorescence and line immunoassay both effectively detect antinuclear antibodies, supporting diagnosis.
• The study shows a significant correlation between antinuclear antibody results and autoimmune disease presence.
• This retrospective cross-sectional study analyzed serum samples from patients suspected of autoimmune disease.
• Findings highlight the importance of choosing appropriate diagnostic methods for accurate autoimmune disease assessment.