Development of a rapid and portable detection method for canine distemper virus based on CRISPR-Cas13a

Key Points

• The detection method successfully identifies canine distemper virus in samples in under one hour, allowing for prompt intervention.
• Using CRISPR-Cas13a technology, the method offers portable testing capabilities for field use, enhancing veterinary practices.
• Analysis of canine samples reveals high sensitivity and specificity, demonstrating effectiveness in detecting viral presence.
• The approach may enable rapid diagnostics in remote areas, highlighting the need for accessible veterinary healthcare solutions.