Sustainable spectrofluorimetric determination of berberine in dietary supplements via Erythrosin B Ion-Pair complexation with mechanistic investigation, Box-Behnken optimization, and green chemistry assessment

Key Points

• Berberine detection is possible with a limit of detection at 0.01 mg/L, enabling precise measurement for dietary supplements.
• The study used Box-Behnken optimization to enhance sensitivity and accuracy in detecting berberine via ion-pair complexation.
• Spectrofluorimetric analysis incorporated green chemistry principles, promoting environmentally friendly practices in chemical analysis.
• Results highlight the potential for sustainable testing methods that reduce harmful waste by using less toxic reagents.