Aptamer-based CRISPR/Cas12a system for amplification-free live pathogenic Shigella flexneri detection

Key Points

• Detection focuses on the pathogenic bacterium Shigella flexneri, which is known for causing dysentery.
• A high specificity observed with the aptamer-based CRISPR/Cas12a system enhances accurate diagnostics in clinical settings.
• Methodology employs an amplification-free approach, streamlining the detection process of bacterial pathogens.
• This approach may enable rapid diagnosis and improve public health response to Shigella outbreaks.