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March 6, 2026Marine Biotechnology0 citationsOpen Access

Genome Size and Karyotype of the Spotted Wolffish (Anarhichas minor)

SPStefano PeruzziLGLaura GhigliottiTVTerese Vollstad-Giæver

Key Points

  • To determine the genome size and karyotype of the spotted wolffish (Anarhichas minor) for future genomic studies.
  • Conducted flow cytometric analysis to measure haploid nuclear DNA content.
  • Performed karyotyping to identify chromosome number and structure.
  • Analyzed any potential chromosomal differences based on sex.
  • Measured average haploid nuclear DNA content of 0.68 pg (665 Mb).
  • Found a diploid number (2n) of 48 chromosomes.
  • Observed no significant differences between male and female chromosome counts.

Abstract

In this study, we report the genome size and karyotype of the spotted wolffish (Anarhichas minor), a species classified at risk in Canada and not commonly the target of directed commercial fisheries elsewhere but increasingly significant for aquaculture. Despite the scientific and commercial interest, there is a lack of cytogenetic and genomic information about this species. Flow cytometric analysis determined an average haploid nuclear DNA content of 0.68 pg (665 Mb), with no significant differences observed between male and female fish. Karyotyping revealed a diploid number (2n) of 48 chromosomes, predominantly consisting of two-armed chromosomes, with no evidence of sex-linked chromosomal heteromorphism. These findings provide critical baseline data for interpreting and validating genome sequencing efforts and lay the groundwork for further cytogenetic studies of A. minor.

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Cite This Study

Peruzzi et al. (2026) studied this question.

synapsesocial.com/papers/69aa70d6531e4c4a9ff5b0f0https://doi.org/10.1007/s10126-026-10586-6
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