Isolation and identification by real time pcr and determination of some antibiotic resistance of Vibrio parahaemolyticus from shrimps

This study was carried out to determine the presence of Vibrio parahaemolyticus (V. parahaemolyticus) from shrimps (n=90) obtained from fishermen in Aydın province. According to traditional methods, 40 isolates gave suspicious positive results on Thiosulfate Citrate Bile Sucrose (TCBS) agar. All V. parahaemolyticus strain known to have thermolabile hemolysin encoded by tlh gene as species marker. In order to identify suspicious strains, tlh genes specific to V. parahaemolyticus were detected by using tlh primers. Eleven strains of the suspicious isolates gave a positive reaction with Real Time PCR. Antibiotic resistance of the isolates gave a positive reaction as V. parahaemolyticus by real-time PCR, penicillin G (10units), clindamycin (2µg), piperacillin (100µg), amoxicillin-clavulanic acid (30µg), ciprofloxacin (5µg) and gentamicin (10µg) was determined by disk diffusion. All isolates showed resistance to Penicillin G and Clindamycin and One isolate showed multiple resistance to all antibiotics tested. All isolates, except the isolate showing multiple antibiotic resistance, were susceptible to Piperacillin, Ciprofloxacin and Gentamicin. As a result, it is recommended to establish effective control measures to reduce the risk of V. parahaemolyticus infection in terms of public health, it is recommended to use effective methods routinely to ensure the safety of foods and to detect V. parahaemolyticus in foods and the environment.