What question did this study set out to answer?

The study aims to explore how the Radix Astragali-Rhizoma Coptidis couple influences liver fat deposition via the Keap1/Nrf2/GPX4 pathway in rats with impaired glucose tolerance.

March 6, 2026Lishizhen medicine and materia medica research0 citations

Radix Astragali-Rhizoma Coptidis medicinal couplet alleviates hepatic fat deposition in rats with impaired glucose tolerance via the Keap1/Nrf2/GPX4 pathway

View Full Paper

RZRuihe Zhu WDWenjie DAI RXRui Xu

Key Points

The study aims to explore how the Radix Astragali-Rhizoma Coptidis couple influences liver fat deposition via the Keap1/Nrf2/GPX4 pathway in rats with impaired glucose tolerance.
Used male Wistar rats on a high-fat, high-sugar diet to create an impaired glucose tolerance model.
Divided rats into three groups: control, Radix Astragali-Rhizoma Coptidis treated, and metformin treated.
Administered interventions for four weeks and assessed general condition, blood glucose, and liver morphology.
Measured protein and mRNA levels of Keap1, Nrf2, HO-1, and GPX4 in liver tissues using immunofluorescence and qPCR.
The herbal treatment significantly reduced blood glucose levels during the OGTT in the treated group (P<0.05).
It decreased liver fat vacuolation and downregulated Keap1 protein and mRNA expression.
Nrf2 protein levels increased, along with the mRNA levels of Nrf2, GPX4, and HO-1, all statistically significant (P<0.05).

Abstract

目的探讨黄芪-黄连药对可能通过Keap1/Nrf2/GPX4通路来降低糖耐量减低大鼠（IGT）肝脏脂肪沉积的潜在作用机制。方法 SPF级雄性Wistar大鼠，随机挑选10只作为空白组，余给予高糖高脂饮食10周和小剂量链脲佐菌素复制IGT模型，分为模型组（n=9）、黄芪-黄连药对组（4.05 g·kg-1·d-1，n=10）、二甲双胍组（0.05 g·kg-1·d-1，n=9）。干预4周，观察各组一般情况、血糖相关指标改变；HE染色观察肝脏组织形态；免疫荧光染色与qPCR检测肝脏组织Keap1、Nrf2、HO-1、GPX4蛋白、mRNA含量。结果干预后，黄芪-黄连可降低OGTT试验2h血糖（PP 结论黄芪-黄连药对可有效降低IGT大鼠血糖和肝脏脂肪空泡形态，其机制可能与调控Keap1/Nrf2/GPX4信号通路有关。

Ask AI

Helpful

Bookmark

View Full Paper

Cite This Study

Zhu et al. (2026) studied this question.

synapsesocial.com/papers/69aa7160531e4c4a9ff5b7fd https://doi.org/10.70976/j.1008-0805.szgygy-2026-0110

Ask AI

Helpful

Bookmark

View Full Paper

March 6, 2026Lishizhen medicine and materia medica research0 citations

Radix Astragali-Rhizoma Coptidis medicinal couplet alleviates hepatic fat deposition in rats with impaired glucose tolerance via the Keap1/Nrf2/GPX4 pathway

View Full Paper

RZRuihe Zhu WDWenjie DAI RXRui Xu

Key Points

The study aims to explore how the Radix Astragali-Rhizoma Coptidis couple influences liver fat deposition via the Keap1/Nrf2/GPX4 pathway in rats with impaired glucose tolerance.
Used male Wistar rats on a high-fat, high-sugar diet to create an impaired glucose tolerance model.
Divided rats into three groups: control, Radix Astragali-Rhizoma Coptidis treated, and metformin treated.
Administered interventions for four weeks and assessed general condition, blood glucose, and liver morphology.
Measured protein and mRNA levels of Keap1, Nrf2, HO-1, and GPX4 in liver tissues using immunofluorescence and qPCR.
The herbal treatment significantly reduced blood glucose levels during the OGTT in the treated group (P<0.05).
It decreased liver fat vacuolation and downregulated Keap1 protein and mRNA expression.
Nrf2 protein levels increased, along with the mRNA levels of Nrf2, GPX4, and HO-1, all statistically significant (P<0.05).

Abstract

Ask AI

Helpful

Bookmark

View Full Paper

Cite This Study

Zhu et al. (2026) studied this question.

synapsesocial.com/papers/69aa7160531e4c4a9ff5b7fd https://doi.org/10.70976/j.1008-0805.szgygy-2026-0110

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

Ask AI

Helpful

Bookmark

View Full Paper