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March 7, 2026Cancer Immunology Research

Abstract LB-C006: Scalable TCR synthesis and screening enable antigen reactivity mapping

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Authors

SGStephanie GaglioneRMRachit S. MukkamalaCKChirag Krishna

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Overview

Innovative method synthesizes and screens TCRs to identify antigen reactivity in tumors, suggesting new therapeutic avenues.

Key Points

  • The aim is to develop a scalable approach for synthesizing and screening T cell receptors to map their antigen targets effectively.
  • Introduced TCRAFT for cost-effective TCR synthesis while maintaining TCRα-β pairing.
  • Integrated TCRAFT with RAPTR for large-scale antigen screening.
  • Reconstructed 3,808 TCRs from vitiligo blister fluid and screened them against hundreds of antigens.
  • Synthesized and screened over 30,800 TCRs from pancreatic ductal adenocarcinoma patients.
  • Identified over 160 antigen-specific TCR clonotypes from the screening process.
  • Established transcriptomic signatures of autoreactive T cells associated with vitiligo.
  • Correlated TCR specificity with tumor-infiltrating lymphocytes in melanoma.

Cite This Study

Gaglione et al. (2026) studied this question.

synapsesocial.com/papers/69abc2855af8044f7a4ec328https://doi.org/10.1158/2326-6074.io2026-lb-c006
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Abstract 8: Deep Immunomics pipeline for discovery and validation of novel cancer-specific T cell receptors2024
  2. 2Abstract 476: A practical workflow for adaptive immune receptor profiling and screening of antigen-specific clonotypes with applications in cancer.2026
  3. 3Abstract 6515: High-throughput, 96-well plate-based single-cell TCR sequencing for scalable chain-pairing and immunophenotyping of cancer-associated T cells2026
  4. 4Abstract 14: Identification of tumor-reactive T cell receptors through functional single cell interaction analyses for personalized T cell therapy2024
  5. 5Abstract 4196: Predicting self-antigen recognition of TCRs derived from tumor infiltrating CD8+ T cells via contrastive learning and T cell phenotyping2026