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March 13, 20260 citationsOpen Access

D1.1 Design, characterisation and optimization of aptamers, ADN/DNA probes and antibodies

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LLl'Alimentation et l'Environnement Institut National de Recherche pour l'AgricultureNANational Technical University of Athens

Key Points

  • The research aims to identify effective bioreceptors for specific organic pollutants using electrochemical biosensors.
  • Developed electrochemical biosensors to detect organic pollutants including pathogenic bacteria and hormones.
  • Screened various bioreceptors including aptamers, antibodies, and DNA probes for effectiveness.
  • Collaborated with multiple partners for comprehensive evaluations.
  • Identified specific bioreceptors capable of binding to target pollutants.
  • Demonstrated varying efficacy among tested bioreceptors for different pollutants.

Abstract

Electrochemical biosensor are based on electronic transducers, capable of providing selective and quantitative analytical information on the electron transfer variations due to interaction with the analyte, and bioreceptors. A bioreceptor serves as a recognition element that binds or transforms the analyte, while the electronic transducer converts the event of bioreceptor–analyte interaction into an electrical signal.MOBILES partners are engaged on developing electrochemical biosensors to detect organic pollutants like pathogen bacteria -Bacillus cereus and Staphylococcus aureus-, antibiotic resistant gene, pesticides and the hormone 17β-estradiol.To identify the best bioreceptor for each pollutant category several molecules such as aptamers, antibodies, and DNA probes must be evaluated and validated.Deliverable D1.1 reports the screening work performed in WP1 by INRAE, NTUA, UBE and UBx aimed at the identification of bioreceptors able to specifically recognize and bind the target pollutants mentioned above. Once identified, within the electrochemical biosensors, such bioreceptors are coupled with the electronic transducer which make them able to quickly identify target biotic and abiotic pollutions. Performance of electrochemical biosensors will be presented in a separate deliverable (D1.4).Due to confidential data disclosure in the document, this deliverable is classified as sensitive. Therefore, only this brief summary is available for the general public.

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Cite This Study

l'Agriculture et al. (2026) studied this question.

synapsesocial.com/papers/69b3ad1302a1e69014ccf655https://doi.org/10.5281/zenodo.18958562
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