Cognitive stress, also known as mental workload, constitutes a central topic within the field of psychophysiology due to its role in modulating attention, autonomic regulation, and stress reactivity. Furthermore, it bears direct relevance to practical monitoring systems that employ non-invasive sensing techniques. This study investigates whether a multimodal, non-invasive measurement setup can detect systematic physiological differences between Resting periods and short episodes of cognitive load within the same individuals. Additionally, it explores the capacity of such a system to differentiate tasks characterized by varying cognitive demands. A sequential, within-subject protocol was employed, comprising five consecutive phases (rest 1, Stroop, rest 12, subtraction, rest 3), during which five modalities were recorded concurrently: EEG, heart rate (HR), galvanic skin response (GSR), facial surface temperature, and oxygen saturation (SpO2). Beyond phase-wise inspection of time-series data, an exploratory assessment of similarity across participants was conducted using correlation coefficients. The maximum cross-participant correlations observed were 0. 88 (HR), 0. 90 (GSR), 0. 83 (facial temperature), and 0. 77 (SpO2) ; however, these correlations were used only as exploratory descriptors of inter-individual similarity and did not imply a significant phase effect. For inferential analysis, phase-wise epoch means were evaluated through one-factor repeated-measures ANOVA. The heart rate exhibited a robust main effect of phase (F (4, 32) = 10. 5862, pGG = 0. 01044, ηp2 = 0. 5696), with higher HR observed during cognitive load epochs (e. g. , 77. 841 ± 11. 777 bpm at rest 1 versus 83. 926 ± 14. 532 bpm during subtraction). The relatively large standard deviation reflects variability between subjects rather than variability within epochs. Regarding processed baseline-referenced GSR, the omnibus phase effect was not statistically significant under the conservative Greenhouse–Geisser correction; therefore, GSR was interpreted as exploratory in this dataset. Facial temperature and SpO2 likewise did not show statistically significant omnibus phase effects under Greenhouse–Geisser correction (e. g. , SpO2: pGG = 0. 1209). EEG-derived measures provide supplementary central evidence of task engagement; entropy variations within an approximate dynamic range of 0. 2 to 0. 8 were observed, and the α/θ ratios demonstrated nearly a twofold distinction between rest and cognitive load epochs across different leads.
Hristov et al. (2026) studied this question.