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March 19, 20260 citationsOpen Access

Functional characterization of CRY-DASH1 and CRY-DASH2 from Chlamydomonas reinhardtii

MSMelvin Schubert

Key Points

  • The central aim is to characterize CRY-DASH1 and CRY-DASH2 in Chlamydomonas reinhardtii and understand their functions.
  • Characterization of two cryptochromes CRY-DASH1 and CRY-DASH2.
  • Intracellular localization studies of the proteins.
  • Development of a new method for intact chloroplast isolation.
  • Generation of complementation mutants for functional analysis.
  • Identified localization and membrane associations of CRY-DASH1 and CRY-DASH2.
  • Discovered metabolic influences linked to the phenotypes of knockout mutants.
  • Generated complementation mutants that partially recover the knockout phenotypes.

Abstract

This thesis is about the characterisation of CRY-DASH1 and CRY-DASH2 - two cryptochromes of Chlamydomonas reinhardtii. It contains findings about the intracellular localization of the proteins, their associations to membranes and their metabolic influences resulting in the discovered phenotypes of the two confirmed knock out mutants. Furthermore, this thesis introduces a new method of intact chloroplast isolation from C. reinhardtii strains containing cell walls as well as generated complementation mutants, which partially recover the phenotype of the knockout mutants.

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Cite This Study

Melvin Schubert (2025) studied this question.

synapsesocial.com/papers/69bb92d1496e729e629805cehttps://doi.org/10.22032/dbt.69706
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