A multiplex PCR amplicon (amplicon)-based sequencing method to facilitate the diagnosis of echinococcosis sample

Key Points

• This research aims to develop a multiplex PCR amplicon-based sequencing method for diagnosing human echinococcosis.
• Developed a multiplex PCR method for amplifying specific DNA regions.
• Utilized plasma samples from individuals for testing.
• Applied sequencing technology to analyze the amplified DNA.
• Demonstrated successful amplification of echinococcosis-related genes.
• Improved diagnostic accuracy with the new method compared to standard techniques.