Investigation of the Phytochemical Composition, Antioxidant, and Anti‐Enzyme Activities of Hedlundia tamamschjanae and Hedlundia Roopiana From Erzurum, Türkiye

Plant materials are currently being investigated as natural additives, antioxidant or potential therapeutic agents. In the present study, in vitro antioxidant and anti-enzyme activities, and phenolic and essential oil contents of extracts obtained from flowers, leaves, and fruits of Hedlundia roopiana and Hedlundia tamamschjanae were investigated. Phenolic contents were determined using HPLC-DAD (high-performance liquid chromatography-diode-array detection) while essential oil contents were determined by SPME/GC-MS (solid phase micro extraction/gas chromatography-mass spectrometry). The highest antioxidant activity was found in Hedlundia tamamschjanae leaf extracts for CUPRAC (copper ion reduction potential) and FRAP (ferric (Fe+3) reducing power) analysis. The lowest DPPH (1,1'-diphenyl-2-picrylhydrazyl radical removal activity) activity was calculated for Hedlundia tamamschjanae inflorescences extract. The enzyme inhibitory activities of the extracts obtained from plants were assayed using hCA I-II (human carbonic anhydrase I-II), AChE (acetylcholinesterase) and BChE (butyrylcholinesterase) enzymes and the lowest enzyme concentrations were in Hedlundia tamamschjanae inflorescences extract for hCA I.