Pterocryptis cochinchinensis is a small freshwater catfish distributed across the Southeast Asia and southern China. It plays an important role in maintaining inland aquatic biodiversity and serves as a valuable model for phylogeographic research. However, its genomic resources are still limited. Here, we report a near-complete telomere-to-telomere genome assembly of P. cochinchinensis, generated by combining MGI short reads (40.6 Gb), PacBio HiFi reads (51.9 Gb), Oxford Nanopore ultra-long reads (15.0 Gb), and Hi-C data (88.8 Gb). The final assembly spans 931.7 Mb, with a contig N50 of 31.4 Mb, and was anchored onto 28 chromosomes. Repetitive sequences account for 46.9% of its genome, and 31,353 protein-coding genes were annotated. BUSCO assessment indicated a genome completeness of 96.7%. This high-quality reference genome will represent a valuable resource for conservation genetics, aquaculture improvement, and comparative genomics within Pterocryptis, contributing to biodiversity preservation and the sustainable utilization of freshwater fish resources.
Chen et al. (2026) studied this question.