What question did this study set out to answer?

The study aims to create a detailed atlas of tertiary lymphoid structures (TLSs) in ovarian cancer to understand their maturation and immune functions.

April 5, 2026Cancer Research0 citations

Abstract 2220: A spatially resolved multi-omics atlas of tertiary lymphoid structures in ovarian cancer.

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ZLZhihan Liang AJAda Junquera HEHanna Elomaa

Key Points

The study aims to create a detailed atlas of tertiary lymphoid structures (TLSs) in ovarian cancer to understand their maturation and immune functions.
Constructed a spatially resolved multi-omics atlas integrating histopathology, spatial transcriptomics, proteomics, and immunogenomics.
Analyzed tumor samples from ovarian cancer patients using deep learning-assisted segmentation and tissue cyclical immunofluorescence (t-CyCIF).
Performed spatial transcriptomic profiling and analyzed data for differential expression and pathway enrichment.
Used SCORPIUS pseudotime analysis to delineate maturation trajectories of TLSs.
Identified substantial heterogeneity in TLS and lymphoid aggregate (LAgg) abundance across different ovarian cancer histologies.
Established distinct microstructures for immature TLSs (iTLSs) and mature TLSs (mTLSs), with mTLSs containing germinal centers.
Profiles indicated a maturation trajectory from LAggs to iTLSs and then to mTLSs, revealing molecular insights into immune activation.

Abstract

Abstract Tertiary lymphoid structures (TLSs) coordinate localized immune responses in the ovarian tumor microenvironment. However, the maturation transitions of TLS remain poorly understood at both cellular and molecular levels. We constructed a spatially resolved multi-omics atlas that integrates histopathology, spatial transcriptomics, proteomics, and immunogenomics to characterize TLS organization, maturation, and clinical relevance in ovarian cancer. H Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 2220.

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Cite This Study

Liang et al. (2026) studied this question.

synapsesocial.com/papers/69d1fdd4a79560c99a0a423e https://doi.org/10.1158/1538-7445.am2026-2220

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April 5, 2026Cancer Research0 citations

Abstract 2220: A spatially resolved multi-omics atlas of tertiary lymphoid structures in ovarian cancer.

View Full Paper

ZLZhihan Liang AJAda Junquera HEHanna Elomaa

Key Points

The study aims to create a detailed atlas of tertiary lymphoid structures (TLSs) in ovarian cancer to understand their maturation and immune functions.
Constructed a spatially resolved multi-omics atlas integrating histopathology, spatial transcriptomics, proteomics, and immunogenomics.
Analyzed tumor samples from ovarian cancer patients using deep learning-assisted segmentation and tissue cyclical immunofluorescence (t-CyCIF).
Performed spatial transcriptomic profiling and analyzed data for differential expression and pathway enrichment.
Used SCORPIUS pseudotime analysis to delineate maturation trajectories of TLSs.
Identified substantial heterogeneity in TLS and lymphoid aggregate (LAgg) abundance across different ovarian cancer histologies.
Established distinct microstructures for immature TLSs (iTLSs) and mature TLSs (mTLSs), with mTLSs containing germinal centers.
Profiles indicated a maturation trajectory from LAggs to iTLSs and then to mTLSs, revealing molecular insights into immune activation.

Abstract

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Cite This Study

Liang et al. (2026) studied this question.

synapsesocial.com/papers/69d1fdd4a79560c99a0a423e https://doi.org/10.1158/1538-7445.am2026-2220

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