Abstract 4130: Single-cell multi-omic characterization of gastric intestinal metaplasia reveals potential genetic, epigenetic and isoform signatures of lesions at high risk for GC progression

Abstract Worldwide, gastric cancer (GC) is the fifth most common malignancy and the third leading cause of cancer-related deaths. Gastric intestinal metaplasia (GIM) is a precursor lesion of GC. However, only a small number of GIM lesions progress to GC. A key challenge is identifying the genomic, molecular and cellular features of GIM that predict their risk of becoming invasive cancer. Once these features are identified, one could “intercept” patients at high risk for developing GC. We conducted a single-cell multi-omic analysis of endoscopic biopsies of GIM lesions. These patients underwent GC risk evaluation using a two-biopsy approach. We used the pathology results to determine a clinical risk stage based on the Operative Link on Gastric Intestinal Metaplasia Assessment (OLGIM). Clinical stages include I and II which are low risk versus III and IV which are high risk. Each biopsy underwent single cell RNA-seq and single cell assay for transposase accessibility of chromatin (ATAC). Therefore, we had both of these genomic readouts for each cell. In addition, we used single cell long read sequencing to identify transcript isoforms and mutations. Overall, we obtained the following single cell cell features of GIM which included: (1) gene expression, (2) chromatin accessibility, (3) copy number aberrations, (4) somatic variants, and (5) isoform expression. We compared the single cell genomics features between high-risk and low-risk groups. For example, we observed that key genes specifically expressed in intestinal-like stem cells, which are linked to potential gastric cancer-initiating cells—such as CDH17, SI and CPS1—were more highly expressed among the high-risk group, which also exhibited increased chromatin accessibility at the same genes. Furthermore, we detected differential isoform usage of gastric-related genes between the two groups, which implies that isoform changes are related to GC progression. These findings provide genomic, molecular and cellular features of GIMs that are associated with high risk of developing GC. Citation Format: Dongin Lee, Xiangqi Bai, Sue Grimes, Kyungtae Lee, Yan Wang, Charlotte Wong, Anuja Sathe, Ignacio Wichmann, Youlim Kim, Rithika Meka, Renee Long, Allison Im, Billy Lau, Robert Huang, Hanlee P. Ji, . Single-cell multi-omic characterization of gastric intestinal metaplasia reveals potential genetic, epigenetic and isoform signatures of lesions at high risk for GC progression abstract. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 4130.