The musk gland of male forest musk deer (Moschus berezovskii) secretes musk enriched with sex steroid hormones. The testes mainly produce these hormones; however, whether glandular cells can autonomously synthesize them remains unexplored. This study aimed to utilize an in vitro-cultured musk gland cell model to investigate whether musk gland cells possess the capability for autonomous synthesis of sex steroid hormones. We used single-cell RNA sequencing (scRNA-seq), reverse transcription quantitative real-time polymerase chain reaction, and liquid chromatography–mass spectrometry (LC-MS) to verify the steroidogenic potential of musk gland cells. scRNA-seq revealed that during the secretion period, 18 cholesterol and 6 sex steroid hormone biosynthesis genes were significantly expressed in the cells. In vitro experiments demonstrated that these genes were expressed without exogenous cholesterol supplementation. LC-MS analysis confirmed stable synthesis of nine sex steroid hormones. Increasing cholesterol concentration to 20 mg/L significantly upregulated SRD5A3 and AKR1D1, with AKR1C3 expression showing an upward trend. Elevated cholesterol increased several sex steroid hormone levels: pregnenolone, progesterone, 17α-hydroxypregnenolone, androstenedione, androsterone, and etiocholanolone by 4.12-, 1.46-, 33.42-, 2.06-, 3.11-, and 5.65-fold, respectively. These results collectively indicate that the musk glandular cells can synthesize sex steroid hormones de novo and suggest that cholesterol may regulate their biosynthesis in these cells.
An et al. (2026) studied this question.