April 12, 1996Science4,935 citations
In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
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Key Points
- To investigate the effectiveness of a lentiviral vector for gene delivery to nondividing cells.
- Developed a retroviral vector system based on HIV.
- Transduced HeLa cells, rat fibroblasts, and human primary macrophages blocked in the cell cycle.
- Mediate stable gene transfer into terminally differentiated neurons in vivo.
- Successful transduction of heterologous sequences in nondividing cells.
- Demonstrated capability for stable gene transfer in terminally differentiated neurons.
Abstract
A retroviral vector system based on the human immunodeficiency virus (HIV) was developed that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additionally, the HIV vector could mediate stable in vivo gene transfer into terminally differentiated neurons. The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy.
Cite This Study
Naldini et al. (1996) studied this question.