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April 10, 20260 citationsOpen Access

Modulation of Ndc80-Microtubule Interactions Using Supramolecular Ligands

Modulation of Ndc80-microtubule interactions by supramolecular ligands and physiological regulators

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Authors

JNJonas Alexander Neblik

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Overview

Research examines Ndc80 interactions with microtubules, introducing ligands to regulate binding in yeast cells, suggesting new therapeutic strategies.

Key Points

  • This work aims to explore the regulatory mechanisms of Ndc80-microtubule interactions and develop new supramolecular ligands.
  • Introduced the yeast Ndc80 CH-domain for ligand development
  • Developed multivalent tweezer assemblies with varying structures
  • Used TIRF microscopy to assess binding inhibition
  • Conducted NMR titrations and in silico modelling to locate interaction sites
  • Purified N-CH constructs for characterization of N-terminal effects
  • New multivalent tweezers showed high affinity binding to the CH-domain
  • Inhibited full-length Ndc80 complex binding to microtubules in a dose-dependent manner
  • N-CH constructs exhibited different binding capacities due to phosphomimetic mutations
  • Wildtype N-CH bound both microtubules and soluble tubulin, leading to filament formation
  • Regulated interactions may influence polymerization dynamics at kinetochores
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Cite This Study

Jonas Alexander Neblik (2025) studied this question.

synapsesocial.com/papers/69d895ea6c1944d70ce07128https://doi.org/10.17185/duepublico/83250
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