Objectives: This study aimed to evaluate the effect of transplantation of mesenchyme stem cells derived from bone marrow and quercetin on the oxidation markers, hormonal profile of testosterone, and morphometric testis and sperm parameters in adult mice poisoned with the antineoplastic drug cyclophosphamide (CTX). Materials and Methods: All 25 mice were divided into five groups control, CTX group received CTX (150 mg/kg), quercetin + CTX group received IP injections of quercetin (75 mg/kg /daily) for 14 days, and CTX (150 mg/kg), CTX + bone marrow mesenchymal stem cells (BMMSCs) also received CTX and, 24 hours after the administration of CTX, approximately 1×106 BMMSCs were injected carefully into each testis of mice. Quercetin + CTX + BMMSCs group received quercetin and CTX (as in group quercetin + CTX), and BMMSCs were injected into each testis of the animals. Thirty-five days after the last injection of BMMSCs, blood samples were taken to gain serum and assess the testosterone level, and histopathological, biochemical, and sperm parameters analyses were performed. Results: CTX led to testicular tissue damage and reduced the Johnson score and diameters of the seminiferous tube in the CTX group (P<0.05). Also, the level of Antioxidant enzyme and testosterone was notably reduced in the CTX group (P<0.05). The count and sperm quality were decreased significantly in the CTX group (P<0.05). Administration of quercetin led to an increase in the level of testosterone and antioxidant enzymes and improved sperm parameters. Conclusions: The present study shows the administration of quercetin can protect the testicle against CTX-related damage and improve oxidative stress damage and sperm parameters.
Abdi et al. (2025) studied this question.