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April 11, 2026Fermentation0 citationsOpen Access

Impact of Pea Fiber (Pisum sativum L.) on the Viability of Limosilactobacillus reuteri ACC27 and Quality Attributes of Fermented Milk

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NHNisa Nur HacıbayramoğluAtatürk UniversityHAHaktan AktaşAtatürk University

Key Points

  • To investigate how different proportions of pea fiber affect the viability of Limosilactobacillus reuteri and the quality of fermented milk.
  • Produced fermented milks with varying pea fiber concentrations (0%, 0.5%, 1%, 1.5%, 2%)
  • Used Limosilactobacillus reuteri ACC27 and Streptococcus thermophilus 212S for fermentation
  • Monitored viable cell counts and fermentation time
  • Evaluated physicochemical properties and antioxidant capacity
  • Pea fiber increased viable cell counts of Limosilactobacillus reuteri by approximately 1 log CFU/g compared to the control
  • Fermentation time was reduced by about 30 minutes with pea fiber addition
  • Detected various organic acids including malic, lactic, acetic, and benzoic acids in all samples
  • Pea fiber supplementation decreased syneresis and increased viscosity
  • Enhanced total phenolic content and antioxidant capacity of the samples

Abstract

As probiotic microorganisms must remain viable at a certain level throughout the shelf life of fermented foods, various plant-based prebiotics are added to fermented dairy products. Pea (Pisum sativum L.) is a remarkable food source due to its prebiotic properties, high phenolic content and antioxidant capacity. In this study, fermented milks containing different proportions of pea fiber powder (0%, 0.5%, 1%, 1.5% and 2%) were produced using Limosilactobacillus reuteri ACC27, which has probiotic potential, and Streptococcus thermophilus 212S. The addition of pea fiber powder promoted the growth of Limosilactobacillus reuteri ACC27, increasing viable cell counts by approximately 1 log CFU/g compared to the control during storage. In addition, the fermentation time was shortened by approximately 30 min in samples containing pea fiber. Malic (84.07–175.58 mg/kg), lactic (11,670.45–13,791.66 mg/kg), acetic (145.12–240.53 mg/kg) and benzoic acids (17.07–20.34 mg/kg) were detected in all samples. Furthermore, pea fiber supplementation improved physicochemical properties by reducing syneresis and modifying water release behavior, while also increasing viscosity. The addition of pea fiber also enhanced total phenolic content and antioxidant capacity of the samples. The results of the principal component analysis revealed that the addition of pea fiber powder was associated with potentially improved functional attributes and enhanced probiotic viability under the studied conditions.

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Cite This Study

Hacıbayramoğlu et al. (2026) studied this question.

synapsesocial.com/papers/69d9e6b078050d08c1b76fc8https://doi.org/10.3390/fermentation12040189
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