Introduction: Abhaya Gutika is a traditional Ayurvedic formulation referenced in the classical text Rasa Ratna Samucchaya for the management of Krimidanta (dental caries). Despite its historical use, modern analytical data regarding its chemical composition is limited. Methodology: The formulation was subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis using a Clarus 680 GC system with an HP-5MS column to identify its volatile and semi-volatile bioactive compounds. Observation and Results: GC-MS analysis revealed a diverse array of phytochemicals at various retention times. Key compounds identified include 1,2,4-Benzenetriol (22.8%), 6-Acetyl-β-d-mannose (22.1% and 45.5% at different RTs), 5-Hydroxymethylfurfural (9.11%), and various fatty acid derivatives like n-Hexadecanoic acid and methyl esters. These compounds are known for their antimicrobial, antioxidant, and anti-inflammatory properties. Conclusion: The study successfully characterized the chemical profile of Abhayadi Gutika. The presence of potent bioactive molecules supports its traditional use in treating dental caries and suggests that the unique preparation method involving a copper vessel may contribute to its therapeutic efficacy.
Dr. Gazala Hussain2* Varshini Hemannavar1 (2026) studied this question.