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April 17, 2026Rapid Communications in Mass Spectrometry0 citations

Microwave‐Accelerated Proteolysis Minimizes Artificial Deamidation and Enhances Speed for Peptide Mapping of Protein Pharmaceuticals

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YZYi‐Feng ZhengNational Chung Hsing UniversityCTChen‐Yuan TsengNational Chung Hsing UniversityYPYa-ting PanNational Chung Hsing University

Key Points

  • The aim is to reduce artificial deamidation during peptide mapping and improve efficiency in protein pharmaceuticals.
  • Utilized microwave-accelerated proteolysis under optimized Tris buffer conditions
  • Compared digestion times before and after microwave application
  • Analyzed effects on artificial deamidation in IgG and BSA proteins
  • Reduced digestion time from 16-18 hours to minutes
  • Lowered artificial deamidation by approximately 60%-80%
  • Findings in IgG and BSA indicate consistency and reliability for quality assessments

Abstract

Microwave-accelerated proteolysis under optimized Tris buffer conditions minimizes artificial N/Q deamidation during peptide mapping while substantially reducing preparation time. Digestion time decreases from 16-18 h to minutes, lowering deamidation by ~60%-80%. Consistent findings in IgG and BSA improve confidence in CQA assessment and support high-throughput biopharmaceutical quality control.

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Cite This Study

Zheng et al. (2026) studied this question.

synapsesocial.com/papers/69e1cf985cdc762e9d8588f4https://doi.org/10.1002/rcm.70081
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