Genes involved in sexual development have been identified in many teleost fishes, including the turbot, a flatfish of high commercial value in aquaculture. In this species, a major sex determination (SD) Quantitative Trait Locus has been identified, and Sox2 has been proposed as the SD gene. Although RT-PCR, qPCR, and transcriptomic analyses have been performed on turbot gonads, histological studies remain limited. Here, we examined the tissue-specific expression patterns of several sex-related genes in turbot using in situ hybridization on paraffin sections (SISH) of differentiated ovaries and testes of juvenile and adult specimens. Vasa, Foxl2, Amh, Sox2 and Sox17 transcripts were detected in both male and female gonads, whereas Cyp19a1a and Sox19 expression was restricted to the ovary; these results support previous gene expression analyses and suggest a role for these genes in gonadal development and reproductive functions in this species. SISH provides complementary information to molecular analyses by identifying the specific cell types expressing the sex-related genes analyzed in gonadal tissue, thereby offering a more comprehensive understanding of gonadal differentiation in turbot. Comparison with results reported for the gonads of other teleost species revealed similar tissue-specific gene expression patterns during sexual development.
Adrio et al. (2026) studied this question.