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April 22, 2026Sustainability1 citationsOpen Access

Effects of Microalgae (Chlorella ZJ and Anabaena azotica) Application on Soil Carbon and Nitrogen Fractions in a Degraded Purple Soil: A Laboratory Incubation Study

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XZXiangbo ZouJCJiong ChengJCJun Cheng

Key Points

  • This study aims to evaluate the effects of microalgae on soil carbon and nitrogen fractions in degraded purple soil.
  • Conducted a 105-day laboratory incubation experiment with Chlorella ZJ and Anabaena azotica application.
  • Measured changes in soil pH, moisture content, carbon, and nitrogen fractions.
  • Analyzed the impact on soil structure and mineralization capacities.
  • Active microalgae significantly increased dissolved organic carbon and nitrogen by up to 6.41 and 5.81 times, respectively.
  • Total organic carbon rose by 147.07% with active treatment compared to control.
  • Soil nitrogen mineralization capacities were enhanced significantly under microalgae application.

Abstract

Enhancing soil nutrient content is fundamental to the ecological restoration of degraded soils. The application of microalgae represents a sustainable approach for soil remediation, as it contributes to environmental CO2 sequestration while recycling nutrients into degraded ecosystems. Through a 105-day laboratory incubation experiment, this study investigated the impact of applying a mixed microalgal suspension containing active/inactive Chlorella ZJ and Anabaena azotica on the C and N fractions of an alkaline, degraded purple soil. The results showed that both active and inactive microalgae treatments (AM and IM) significantly decreased soil pH and increased soil moisture content (SMC). The AM treatment notably increased the proportion of large soil aggregates and enhanced soil structure. Both treatments significantly enhanced soil C and N fractions: dissolved organic carbon/nitrogen (DOC/DON) increased by 6.41/5.81 times (AM) and 4.22/4.76 times (IM) that of the control (without microalgae application); total organic carbon (TOC) rose by 147.07% (AM) and 138.73% (IM); and the contents of coarse particulate and mineral-associated organic C and N were also significantly elevated. Total nitrogen (TN) significantly increased only under the AM treatment. Soil C and N mineralization capacities were enhanced by 1.01–1.34 times and 7.56–8.43 times that of the control, respectively, indicating a more pronounced stimulation of N mineralization. Fluorescence analysis revealed that both AM and IM treatments increased the complexity and humification of dissolved organic matter. The application of microalgae significantly improved the soil structure and chemical characteristics of the degraded soil and enhanced the C/N pools, thereby creating favorable conditions for soil restoration.

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Cite This Study

Zou et al. (2026) studied this question.

synapsesocial.com/papers/69e865476e0dea528dde9da0https://doi.org/10.3390/su18084057
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