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April 30, 2026Cell Proliferation0 citationsOpen Access

Determination of ATP Content in Cells

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BFBoqiang FuWHWenfeng HuangYLYing Liu

Key Points

  • The aim is to quantify ATP levels in cells using HPLC techniques.
  • HPLC analysis of ATP standard and cellular ATP
  • Use of Poroshell 120 EC-C18 column
  • Chromatograms compared for standard and cellular samples.
  • ATP content was successfully quantified in cellular samples through HPLC.
  • The chromatograms provided clear distinction between ATP standard and cellular levels.

Abstract

The overlap HPLC chromatograms of ATP standard (red) and ATP in cells (blue). Poroshell 120 EC-C18 (3 × 150 mm, 2.7 μm), 25°C, pH 6.8 buffer solution of 0.05 mol/L KH

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Cite This Study

Fu et al. (2026) studied this question.

synapsesocial.com/papers/69f2f0e31e5f7920c6386e06https://doi.org/10.1111/cpr.70184
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