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May 1, 20260 citationsOpen Access

Comparative Study on the Microbiological and Physico-Chemical Properties of Spring Water and Tap Water

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NTNjoku-Obi TreasureImo State University

Key Points

  • This study compares the microbiological and physico-chemical properties of spring water and tap water to assess their safety for consumption.
  • Samples were serially diluted and cultured on nutrient agar, MacConkey agar, Mannitol salt agar, and SDA using the pour plate method.
  • Analysis included total heterotrophic bacteria count, total coliform count, and identification of bacterial isolates.
  • Physico-chemical properties were evaluated against WHO standards.
  • Total heterotrophic bacteria count ranged from 6.0×10^3 to 3.0×10^4 CFU/ml across samples.
  • Total coliform count ranged from 3.2×10^3 to 8.0×10^4 CFU/ml, with isolates including Klebsiella spp, Bacillus spp, E. coli, and Pseudomonas spp.
  • Some samples showed no bacterial growth, while others contained bacteria linked to public health concerns.

Abstract

Comparative study on the microbiological and physico-chemical properties of spring water and tap water was carried out, samples were serially diluted and cultured on Nutrient agar, macconkey agar, Mannitol salt agar and SDA using the pour plate method of inoculation. the total heterotrophic bacteria count ranged from 6.0×103 to 3.0×104, the total coliform count ranged from 3.2×103 to 8.0×104 no growth was recorded for staphylococcal count and Total Fungal Count for the samples ranged from 3.0×103 to 6.0×104. It was found that the isolates belonged to Klebsiella spp, Bacillus spp, E. coli and Pseudomonas spp. Bacillus spp had a percentage distribution of 19, while Klebsiella spp had a percentage occurrence of 24, also Pseudomonas spp had a percentage distribution of 24 and E. coli had a percentage distribution of 33 Physico-chemical analysis showed that the physic-chemical parameters where within the range of WHO standard. This study revealed that in all the water samples analyzeed some had bacteria loads though some did not exhibit the presence of bacteria and the bacteria isolated are of public health importance as they have being linked to disease outbreak and can cause severe health challenges to mankind if there is continued intake Residents who use sampled water as their source of drinking water should apply some purification or water treatment techniques like boiling before drinking so that the pathogenic bacteria isolated can be killed before consumption

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Cite This Study

Njoku-Obi Treasure (2024) studied this question.

synapsesocial.com/papers/69f44488967e944ac55677dchttps://doi.org/10.5281/zenodo.19896197
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