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May 3, 20260 citations

HPTLC and LC-MS Based Metabolomics and Network Pharmacology for Garlic (Allium sativum) and Jamun (Syzygium jambolanum).

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MSManju SinghGGauravPGPraveen Kumar Gaur

Key Points

  • This research aims to explore the bioactive substances in garlic and jamun and their effects on inflammation-related pathways.
  • Employs HPTLC and LC-MS for metabolomic analysis of A. sativum and S. jambolanum.
  • Conducts network pharmacology analysis to identify protein interactions and pathways.
  • Utilizes DisGeNET mapping to relate findings to disease conditions.
  • Identified key bioactive substances like quercetin and kaempferol interacting with IL-1β.
  • Showed involvement in inflammation and microbe-related conditions such as tubulointerstitial fibrosis.
  • Suggests plant-derived therapeutics based on traditional medicinal uses.

Abstract

values, and LC-MS analysis showed key bioactive substances including ferulic acid, gallic acid, naringenin, catechin, quercetin, and myricetin. The Network pharmacology study identified interactions with key proteins, including IL-1β, TLR4, IL6, NOS3, ABCA1, with prominent interaction of quercetin and kaempferol with the protein IL-1β. The DisGeNET mapping findings point toward their participation in key pathophysiological conditions like inflammation, interstitial inflammation, myofibroblast activation causing tubulointerstitial fibrosis, and microbial infection. The study concludes that A. sativum and S. jambolanum actively play a significant role in inflammation and associated dysfunctions, hence providing novel plant-based therapeutics as A. Sativum and S. jambolanum by highlighting the significance in traditional medicine as well as their potential relevance in contemporary pharmacology.

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Cite This Study

Singh et al. (2026) studied this question.

synapsesocial.com/papers/69f6e62e8071d4f1bdfc6c62https://doi.org/10.1002/bmc.70475
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