Phytochemical screening and In-vivo/ In-vitro bioactivity of Rhamnus alaternus vs Tuta absoluta

In in-vitro and in-vivo bioassays, we assessed the insecticidal and antifeedant activity against of aqueous extract of Rhamnus alaternus L. (Rhamnaceae) leaves against Tuta absoluta Meyrick (Lepidoptera: Gelechiidae). We studie the insecticidal and antifeedant activity of aqueous (10.44 %) and ethanolic (12.9 7%) maceration extracts against Tuta absoluta and identified their phytochemicals (Flavonoids, tannins, saponins, alkaloids, sterols and terpenoids). The in- vitro 20 % aqueous extract proved significantly lethal (100 %) and antifeedant (53.08  1.70 %) bioinsecticide potential. 20 days after treatment, in in-vivo trials of curative, preventative and reference treatments based on Chlorantraniliprole/ Abamectin showed mortality of 70 %, 54.8 % and 96.4 %, respectively, with reduction in the damage index, showed quicker effects. The aqueous extract demonstrated their lethal and anti-feedent bio-insecticide potential, ideal for integrated pest management against T. absoluta, with fast action and an environmentally friendly profile.