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May 11, 2026Materials Today Sustainability0 citationsOpen Access

Process Optimization and Characterization of Cottonseed Biodiesel using Box Behnken Design and Artificial Neural Network Approaches

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YMYohannes Kefale MangeshaRNRamesh Babu NallamothuVAVenkata Rammayya Ancha

Key Points

  • This study aims to optimize the yield of biodiesel from cottonseed oil using Box Behnken Design (BBD) and Artificial Neural Network (ANN) methods.
  • Experimental setup included varying methanol to oil ratios, KOH catalyst concentrations, and reaction times.
  • BBD designed the experimental matrix while ANN predicted biodiesel yield with a specific architecture.
  • Model accuracy validation included gas chromatography-mass spectrometry analysis of the biodiesel produced.
  • Maximum biodiesel yield of 96.71% achieved through BBD at optimal conditions.
  • ANN demonstrated superior model performance with R² of 99.3, MSE of 72, and RMSE of 26.8.
  • Biodiesel met ASTM D651 and EN 14214 standards, confirming its potential for sustainable energy.

Abstract

The production of biodiesel requires effective and predictive optimization strategies due to the growing demand for sustainable energy and the environmental constraints of fossil diesel. The present study was amid optimize biodiesel yield from cottonseed feed stock using BBD and ANN methods. The combined of statistical interpretability of BBD with the nonlinear predictive capability of ANN, were enhanced the reliability of optimization and prediction accuracy. The effects of methanol to oil ratio (3:1-9:1), KOH catalyst concentration (1-2 wt. %), and reaction time (60-80 min) on biodiesel yield were systematically investigated. BBD was used to design the experimental matrix and evaluate parameter interactions, while ANN with a 3-10-1 architecture was applied to predict biodiesel yield and assess model accuracy. The maximum cottonseed oil methyl ester yield was obtained at a methanol to oil ratio of 6:1, 1 wt. % of KOH, and 60 min reaction time. Biodiesel yields of 95.5% by experimental, 96.71% by BBD, and 95.48% ANN were attained, thereby validating the efficacy and reliability of the proposed dual optimization approach. Gas Chromatography-Mass Spectrometry (GC-MS) analysis confirmed methyl linoleate as the dominant ester (44.06%), and the produced biodiesel satisfied ASTM D651 and EN 14214 fuel standards. The model was confirmed P-value of 0.00 with Adj R 2 , R 2 , of 98.6 and 98.7% respectively. ANN surpassed BBD by achieving a higher coefficient of determination (R 2 = 99.3) alongside lower mean squared error (MSE = 72) and root mean squared error (RMSE = 26.8) in contrast to BBD (R 2 = 98.6, MSE = 81, RMSE = 28.5). The findings demonstrate that the combined BBD-ANN framework is a robust and viable tool for biodiesel process optimization, offering high efficiency, reduced processing time, and strong potential for environmentally sustainable fuel production.

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Cite This Study

Mangesha et al. (2026) studied this question.

synapsesocial.com/papers/6a0171983a9f334c28271c97https://doi.org/10.1016/j.mtsust.2026.101382
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