Fluorescence of Dyes Adsorbed on Highly Organized, Nanostructured Gold Surfaces

It is shown that fluorescent dyes can be adsorbed selectively on gold nanoparticles which are immobilized on a glass substrate and that the fluorescence originating from the adsorbed dyes exhibits significantly less quenching when compared to dyes adsorbed on bulk gold. Self-assembled monolayers of lissamine sulfide molecules have been studied both on bulk gold and on glass surfaces bearing gold nanoparticles. Gold nanoparticles have been arranged in ordered, two-dimensional patterns, with periodicity in the microm range and used as substrate for the fluorescent dyes. Optical resolution of the fluorescence originating from the pattern has been achieved with laser-scanning confocal microscopy.