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September 1, 1969Journal of Biological Chemistry74 citationsOpen Access

The Composition and Structure of the Carbohydrate of Pineapple Stem Bromelain

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JSJane R. ScoccaYLY. C. Lee

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Abstract

Abstract Two major components of pineapple bromelain, purified to electrophoretical homogeneity, appeared to have the same oligosaccharide group consisting of d-glucosamine, d-mannose, d-xylose, and l-fucose in ratios of 2:2:1:1. By exhaustive proteolysis of the bromelains, glycopeptides containing only Asx, Glx, and Ser were obtained. Periodate oxidation, methylation, and glycosidase digestion showed that the oligosaccharide chain has a highly branched structure in which all the neutral sugars are in nonreducing terminal positions and both N-acetyl-d-glucosamine residues occur in internal positions.

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Cite This Study

Scocca et al. (1969) studied this question.

synapsesocial.com/papers/6a092fef1d1abd907d160d54https://doi.org/10.1016/s0021-9258(18)94282-1
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