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May 17, 2026Journal of Biological Chemistry0 citationsOpen Access

Quantitative FRET FLIM imaging reveals multiple interactions between proteins of the ESYT, ORP and VAP families at the endoplasmic reticulum membrane

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DZDounia ZamiatiMAMouna AbdesselemONOliver Nüße

Key Points

  • To explore protein interactions within the ESYT, ORP, and VAP families at the endoplasmic reticulum membrane.
  • Quantitative FRET FLIM imaging used to assess protein interactions
  • Focus on clustering behaviors among specific protein pairs
  • Analysis of FRET efficiency in relation to potential binding partners.
  • Identified novel clustering between VAPB and ORP8, and ESYT2 with ORP5/8
  • Demonstrated a broad interaction network for VAPA/B
  • Showed that binding partners can significantly influence FRET efficiency.

Abstract

concentration, as observed for ESYT1. We also identify novel inter-family organization, including clustering between VAPB and ORP8, and between ESYT2 and ORP5/8. Finally, our approach highlights the broad interaction network (interactome) of VAPA/B, and shows how various potential binding partners can influence FRET efficiency.

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Cite This Study

Zamiati et al. (2026) studied this question.

synapsesocial.com/papers/6a095a427880e6d24efe0604https://doi.org/10.1016/j.jbc.2026.113144
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