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May 17, 2026Inorganic Chemistry3 citations

Amino-Functionalized Zn(II)-Based Trilinker Metal–Organic Frameworks Conjugated with Folic Acid for Targeted Delivery of Doxorubicin on the MCF-7 Cell Line of Human Breast Cancer

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RAReza AbazariUniversity of MaraghehZYZahra YaghoubiUniversity of MaraghehJGJoanna GościańskaAdam Mickiewicz University in Poznań

Key Points

  • This study aims to develop a stable and efficient drug carrier for targeted delivery of doxorubicin using a novel metal-organic framework.
  • Designed a pH-responsive NH2–Zn-MUM-11 metal-organic framework conjugated with folic acid.
  • Tested biocompatibility on normal breast epithelial cells (MCF-10A) and cytotoxicity on MCF-7 breast cancer cells.
  • Evaluated drug release under different pH conditions and utilized flow cytometry and confocal microscopy for cellular uptake analysis.
  • FA@NH2–Zn-MUM-11 showed 97% drug release at pH 5.2 compared to 24% at physiological pH (p<0.01).
  • Demonstrated no cytotoxic effects in MCF-10A cells, while significant cytotoxicity observed in MCF-7 cells.
  • Efficient folate receptor-mediated uptake led to effective cancer cell elimination.

Abstract

The development of stable, safe, and efficient carriers for targeted delivery of active pharmaceutical ingredients is a key focus in contemporary inorganic, medicinal, and materials chemistry. In the present study, a pH-responsive carrier was rationally designed based on an amino-functionalized Zn (II) trilinker metal–organic framework (MOF), Zn3 (DAP) (BTC) 6 (BTA) 3n•4. 425nDMF, abbreviated as NH2–Zn-MUM-11 MUM = Material from the University of Maragheh, 3, 5-diaminopyridine (DAP), 1, 3, 5-benzenetricarboxylic acid (BTC), benzotriazole (BTA), and conjugated with folic acid (FA), to enable selective delivery of doxorubicin (DOX) as a model anticancer drug. The FA@NH2–Zn-MUM-11 carrier demonstrated high biocompatibility, with no cytotoxic effects observed in normal breast epithelial cells (MCF-10A), while inducing significant cytotoxicity in human breast cancer cells (MCF-7). Under acidic conditions (pH 5. 2), the system achieved nearly a 4-fold increase in drug release relative to physiological pH (97% vs 24%, respectively). Flow cytometry, the wound healing assay, and confocal laser scanning microscopy (CLSM) collectively demonstrated the system’s ability to recognize cancer cells via folate receptor-mediated uptake, facilitate intracellular DOX release, and promote efficient cancer cell elimination. Hence, the obtained material might be further explored as a promising alternative for drug delivery.

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Cite This Study

Abazari et al. (2026) studied this question.

synapsesocial.com/papers/6a095ac47880e6d24efe0a49https://doi.org/10.1021/acs.inorgchem.6c01291
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