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January 1, 1987Nucleic Acids Research850 citationsOpen Access

Electroporation for the efficient transfection of mammalian cells with DNA

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GCGilbert ChuStanford Cancer InstituteHHHiroshi HayakawaMuseum of Natural and Environmental History, ShizuokaPBPaul BergUniversité de Bretagne Sud

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Abstract

A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.

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Cite This Study

Chu et al. (1987) studied this question.

synapsesocial.com/papers/6a09f50516dfdfe7ed348119https://doi.org/10.1093/nar/15.3.1311
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