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May 18, 2026Journal of Biological Chemistry1 citationsOpen Access

Acid Sensing Ion Channel 2a assembles with Epithelial Na+ Channel β and γ subunits to form mechanosensitive ion channels

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SSShujie ShiSWSarah Christine WhelanKSKennedy Szekely

Key Points

  • The aim is to investigate how ASIC2a interacts with ENaC β and γ subunits to form mechanosensitive ion channels.
  • Oocytes were used to express different combinations of ASIC2a and ENaC subunits.
  • Channel activity was measured under varying pH conditions to assess mechanosensitivity.
  • Structural modeling complemented experimental findings to explain interactions.
  • ASIC2aG430V+β/γENaC showed a four-fold increase in channel activity compared to ASIC2aG430V alone.
  • Exposure to acidic pH led to greater activity in ASIC2aG430V+β/γENaC than in ASIC2aG430V alone.
  • ENaC β and γ subunits did not affect the channel's permeability to monovalent cations.

Abstract

currents in oocytes expressing ASIC2aG430V or ASIC2aG430V+βENaC, which was further increased to approximately four-fold when γENaC or both β and γ subunits were co-expressed with ASIC2aG430V (ASIC2aG430V+γENaC or ASIC2aG430V+β/γENaC). Exposure to acidic pH evoked greater channel activity in oocytes expressing ASIC2aG430V+β/γENaC than ASIC2aG430V alone. In contrast, ENaC β and γ subunits did not alter the channel's permeability to monovalent cations of different sizes. Collectively, our experimental findings and structural modeling suggest that ASIC2a assembles with ENaC β and γ subunits to form mechanosensitive channels.

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Cite This Study

Shi et al. (2026) studied this question.

synapsesocial.com/papers/6a0aabc25ba8ef6d83b6f74fhttps://doi.org/10.1016/j.jbc.2026.113151
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