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December 16, 2009359 citationsOpen Access

Quantitative phylogenetic assessment of microbial communities indiverse environments

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CMChristian von Mering

Key Points

  • To develop a quantitative, accurate phylogenetic profiling method for environmental microbial communities using protein-coding marker genes rather than PCR-based ribosomal RNA approaches.
  • Extracted universal protein-coding marker genes from large-scale shotgun metagenomic sequencing datasets across four diverse environments.
  • Mapped the recovered marker genes onto a reference species phylogeny to evaluate community structure, evolutionary rates, and ecological preferences.
  • Showed that rates of sequence evolution vary significantly between microbial communities inhabiting different environments.
  • Identified preferred ecological habitats across entire microbial clades and demonstrated that these habitat preferences remain remarkably stable over evolutionary time.

Abstract

The taxonomic composition of environmental communities is an important indicator of their ecology and function. Here, we use a set of protein-coding marker genes, extracted from large-scale environmental shotgun sequencing data, to provide a more direct, quantitative and accurate picture of community composition than traditional rRNA-based approaches using polymerase chain reaction (PCR). By mapping marker genes from four diverse environmental data sets onto a reference species phylogeny, we show that certain communities evolve faster than others, determine preferred habitats for entire microbial clades, and provide evidence that such habitat preferences are often remarkably stable over time.

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Christian von Mering (2009) studied this question.

synapsesocial.com/papers/6a0ecff137aeb0126447ad8chttps://doi.org/10.1126/science.1133420
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