Vibrio phycocola sp. nov. and Vibrio phycohabitans sp. nov., Isolated from the Phycosphere of Marine Algae

Two Gram-stain-negative, facultatively aerobic, oxidase-and catalase-positive, motile (by means of a polar flagellum) rod-shaped bacterial strains, designated BS-M-Sm-2 T and MA40-2 T , were isolated from marine algae.Growth was optimal at pH 7.0-8.0and 2.0-3.0%(w/v) NaCl, with temperature optima of 25C for BS-M-Sm-2 T and 25-30C for MA40-2 T .Ubiquinone-8 was the sole respiratory quinone.The major fatty acids common to both strains were C 16:0 , summed feature 3 (C 16:1 7c and/or C 16:1 6c), and summed feature 8 (C 18:1 7c and/or C 18:1 6c), while BS-M-Sm-2 T additionally contained C 12:0 and C 14:0 .The predominant polar lipids were phosphatidylethanolamine and phosphatidylglycerol, with diphosphatidylglycerol also detected in strain MA40-2 T .The DNA G+C contents of strains BS-M-Sm-2 T and MA40-2 T were 44.2 and 39.8 mol%, respectively.The 16S rRNA gene sequence similarity, average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH) values between the two strains were 93.8%, 71.4%, and 23.2%, respectively.Phylogenetic and phylogenomic analyses placed both strains within the genus Vibrio, forming distinct lineages.Comparisons with closely related Vibrio type strains yielded ANI and dDDH values below 91.6% and 44.3%, respectively, further supporting their classification as novel species.Genome analyses revealed genes potentially involved in algal symbiosis, including those for polysaccharide degradation and vitamin biosynthesis.Based on comprehensive genomic, phylogenetic, phenotypic, and chemotaxonomic evidence, strains BS-M-Sm-2 T and MA40-2 T represent two novel species, for which the names Vibrio phycocola sp.nov.(BS-M-Sm-2 T =KACC 24066 T =DSM 119941 T ) and Vibrio phycohabitans sp.nov.(MA40-2 T =KACC 24064 T = DSM 119942 T ) are proposed.